Stands for ‘quantitative PCR’, sometimes also known as ‘real-time PCR’. A PCR reaction incorporating a coloured dye that fluoresces during amplification, allowing a machine to track the progress of the reaction. Often used with species-specific Primers where detection of amplification is used to infer presence of the target species’ DNA in the sample. If the species is not present in the sample, no fluorescence will be detected. The high specificity of the qPCR method makes it ideal for situations where a single target is required. The most common use of qPCR testing is for detection of Great Crested Newts from water samples.