Do you ever find new species while doing these surveys? How do you go about identifying them?
This is closely related to the previous question. We frequently encounter taxa where we are unable to make a definitive species-level assignment. This can happen where several species have identical sequences in the region targeted by the assay, or where there are gaps in the reference data. In these cases we make a taxonomic assignment at the lowest level at which we are confident, given the available reference data.
